In vItro flowering of Phyllanthus tenellus roxb. Cultured under different light qualities and growth regulators

Tissue cultures of Phyllanthus tenellus Roxb. were maintained on modified MS to evaluate in vitro flowering under light of different qualities: white (control), blue, green, red, yellow, UV-A plus white and darkness; as well as different growth regulators at concentrations (μM): IBA 0.98, KIN 0.46, KIN 2.3, IBA 0.98 + KIN 0.46, IAA 1.14 + KIN 0.46. The events from vegetative to reproductive growth were observed under in vitro conditions. Flowering was verified from 15 days in all light treatments. Plantlets grown under darkness presented 6% of flowered plantlets within 60 days. White and blue lights showed the highest in vitro flowering percentage (70% and 56%, respectively). A reduction of flowering was observed under red, yellow and green light exposure. IBA added at 0.98 μM concentration induced a greater number of flowers per branch (4.4±0.5). In vitro fruiting was obtained within 60 days.